Technical Name 開發鈀奈米薄膜電化學感測平台用於病毒檢測與抑制分子篩選
Project Operator National Yang Ming Chiao Tung University
Project Host 張家靖
Summary Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infected human cell through ACE2. This study is developing a Pd nano-thin film electrode for electrochemical impedance spectroscopy. This invention can quantify neutralize antibody too. The detection platform can be used to screen 1 microliter anti-virus infection drug sample within 21 mins. Compare to SPR standard, our invention only needs 1/10000 concentration of ACE2reaction volume is ~1/30 than SPR. This invention can be adapted in detection of cancer markers,other emergency infection diseasestherapeutic drugs, too.
Technical Film
Scientific Breakthrough This invention is the novel biosensing platform is based on the interaction between spike proteinACE2 interaction. Therefore, the mutant spike proteins can be analyzed. Furthermore, the titer of neutralize antibody can be detected as well. Conventional ELISA cannot tell neutralize antibody from other binding antibodies. Furthermore, surface active biosensing electrode can direct immobilize ACE2 within 20 minthe volume of protein/drug samples are 1l each. Compare to SPR standard, our invention only needs 1/10000 concentration of ACE2reaction volume is ~1/30~1/100.
Industrial Applicability Surface active Pd nano-thin film based electrochemical platform is the key technique for SARS CoV-2 sensinganti-infection drugs screening. This technique which can conjugate biomolecules directly without linkers. The detection time is as good as SPR gold standard, but with ~1/10000 of concentration~1/30 of volume less than SPR. This invention can quantify neutralize antibody as well. Moreover, this technique can be adapted in detection of cancer markers,other emergency infection diseasestherapeutic drugs, too. Both health impactmarket value will be extremely high.
Matching Needs 1.欲媒合之產業領域:電子與光電、材料化工、生技醫藥、生活應用。2.欲媒合項目:技術轉移、生產製造、投資合作。
Keyword Severe acute respiratory syndrome coronavirus 2 Spike protein Mutant strains of SARS-CoV-2 Angiotensin-converting enzyme 2 (ACE2) Angiotensin Converting Enzyme inhibitors (ACEi) Electrochemical biosensing Metal sputtering Pd nano-thin film electrode Neutralization antibody after vaccination Titers
Notes
  • Contact
  • 張家靖